mouse anti human cd39  (Bio-Rad)

Journal: International Journal of Molecular Sciences

Article Title: Effects of Modified Glucosamine on the Chondrogenic Potential of Circulating Stem Cells under Experimental Inflammation

doi: 10.3390/ijms241210397

Figure Lengend Snippet: Detection of CD39 ( A , C ) and CD73 ( B , D ) expression in CMC cells. The samples were pretreated with TNFα (10 μg/mL) for 3 h and thus cultured for a further 24 h with a proliferative (PM) or chondrogenic (CM) medium supplemented with DCF001 (1 µg/mL). Unstimulated cells (control) or samples treated with only DCF001 or TNFα were used as references. The analysis was performed by flow cytometry using direct staining, and data were reported as representative histograms and relative MFI values (Rel. MFI = target MFI/Isotype IgG MFI). * p : vs. control; ^ p : vs. TNFα-primed cells.

Article Snippet: Mouse anti-human CD39 , Santa Cruz Biotecnology, Inc. (Dallas, TX, USA).

Techniques: Expressing, Cell Culture, Control, Flow Cytometry, Staining

Journal: International Journal of Molecular Sciences

Article Title: Effects of Modified Glucosamine on the Chondrogenic Potential of Circulating Stem Cells under Experimental Inflammation

doi: 10.3390/ijms241210397

Figure Lengend Snippet: Antibodies used for flow cytometry analysis.

Article Snippet: Mouse anti-human CD39 , Santa Cruz Biotecnology, Inc. (Dallas, TX, USA).

Techniques: Flow Cytometry, Control

Journal: Cancer Immunology Research

Article Title: CD161 Characterizes an Inflamed Subset of Cytotoxic T Lymphocytes Associated with Prolonged Survival in Human Papillomavirus–Driven Oropharyngeal Cancer

doi: 10.1158/2326-6066.CIR-22-0454

Figure Lengend Snippet: The phenotype and function of CD161 + CTLs. A, Heat map displaying expression values of discriminative genes between KLRB1 − and KLRB1 + CTLs (from clusters 0, 2, 3, and 9) based on the data from single-cell RNA sequencing. B, Scores for the terminal exhaustion signature, tissue-resident memory T-cell signature, and chemokine/IFNγ signature in KLRB1 − and KLRB1 + CTLs based on the data from single-cell RNA sequencing. C, Coexpression of cytotoxic cytokines and inhibitory receptors on CD161 − or CD161 + CTLs in OPSCC biopsies ( n = 13–19) via flow cytometry, paired Student t test. D, MFI of cytotoxic cytokines coexpressed on CD161 − or CD161 + CTLs treated with PD-1 or CD39 blocking antibodies in OPSCC biopsies ( n = 7–8), paired Student t test. E, MFI of IFNγ coexpressed on CD161 + CTLs treated with CD161-blocking antibodies for 72 hours in HPV + biopsies ( n = 3) and blood samples ( n = 4), paired Student t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n.s.: no statistical significance.

Article Snippet: For the checkpoint blockade experiment, cryopreservated TILs or PBMCs were thawed, expanded, and seeded (5 × 10 5 cells/well) as described, and treated with PD-1 blocking monoclonal antibody (mAb; BioLegend, clone EH12.2H7, cat. #329926, 10 μg/mL), CD39 blocking mAb (Bio-Rad, clone A1, cat. #MCA1268EL, 10 μg/mL), or Tim-3 blocking mAb (BioLegend, clone F28-2E2, cat. #345004, 10 μg/mL) for 7 days.

Techniques: Expressing, RNA Sequencing, Flow Cytometry, Blocking Assay

Journal: Cancer Immunology Research

Article Title: CD161 characterizes an inflamed subset of cytotoxic T lymphocytes associated with prolonged survival in human papillomavirus-driven oropharyngeal cancer

doi: 10.1158/2326-6066.cir-22-0454

Figure Lengend Snippet: Figure 4. The phenotype and function of CD161þ CTLs. A, Heat map displaying expression values of discriminative genes between KLRB1 and KLRB1þ CTLs (from clusters 0, 2, 3, and 9) based on the data from single-cell RNA sequencing. B, Scores for the terminal exhaustion signature, tissue-resident memory T-cell signature, and chemokine/IFNg signature in KLRB1 and KLRB1þ CTLs based on the data from single-cell RNA sequencing. C, Coexpression of cytotoxic cytokines and inhibitory receptors on CD161 or CD161þ CTLs in OPSCC biopsies (n ¼ 13–19) via flow cytometry, paired Student t test. D, MFI of cytotoxic cytokines coexpressed on CD161 or CD161þ CTLs treated with PD-1 or CD39 blocking antibodies in OPSCC biopsies (n ¼ 7–8), paired Student t test. E, MFI of IFNg coexpressed on CD161þ CTLs treated with CD161-blocking antibodies for 72 hours in HPVþ biopsies (n ¼ 3) and blood samples (n ¼ 4), paired Student t test. , P < 0.05; , P < 0.01; , P < 0.001. n.s.: no statistical significance.

Article Snippet: For the checkpoint blockade experiment, cryopreservated TILs or PBMCs were thawed, expanded, and seeded (5 105 cells/well) as described, and treated with PD-1 blocking monoclonal antibody (mAb; BioLegend, clone EH12.2H7, cat. #329926, 10 mg/mL), CD39 blockingmAb (Bio-Rad, clone A1, cat. #MCA1268EL, 10 mg/mL), or Tim-3 blocking mAb (BioLegend, clone F28-2E2, cat. #345004, 10 mg/mL) for 7 days.

Techniques: Expressing, RNA Sequencing, Cytometry, Blocking Assay

Journal: Cell Journal (Yakhteh)

Article Title: Helios, CD73 and CD39 Induction in Regulatory T Cells Exposed to Adipose Derived Mesenchymal Stem Cells

doi: 10.22074/cellj.2020.6313

Figure Lengend Snippet: Morphological characterization of adipose-derived mesenchymal stem cells (ASCs) as well as flow cytometry analysis. A. ASCs were appeared with spindle shape in culture (scale bar: 100 μm) and B. The flow cytometry analysis of ASCs illustrate that more than 90% of all cancer and normal ASCs were positive for stem cell specific markers including CD44, CD105, CD73 and CD90, but they were negative for the expression of hematopoietic specific markers such as CD14, CD45 and CD34.

Article Snippet: Flow cytometry analysis was performed with a FACSCalibur (BD Biosciences) using directly labeled monoclonal Abs (mAbs), PerCP mouse anti-human CD4, FITC mouse anti-human CD25, APC mouse anti-human CD45RA, PerCP-CYTM5.5 mouse anti-human CD73, APC mouse anti-human CD39, PE mouse anti-human FOXP3 (BD Biosciences), APC antimouse/ human Helios (Biolegend, Germany) and isotype control antibodies, all according to the manufacturers’ protocol.

Techniques: Derivative Assay, Flow Cytometry, Expressing

Journal: Cell Journal (Yakhteh)

Article Title: Helios, CD73 and CD39 Induction in Regulatory T Cells Exposed to Adipose Derived Mesenchymal Stem Cells

doi: 10.22074/cellj.2020.6313

Figure Lengend Snippet: Percentage of the CD39 + CD73 + in CD25 + FOXP3 + and CD25 - FOXP3 + T cells population subsequent to co-culturing with adipose-derived mesenchymal stem cells (ASCs). After five days culturing of naïve CD4 + T cells with ASCs, percentage of CD39 + CD73 + T cells in A. CD25 + FOXP3 + population and B. CD25 - FOXP3 + population was evaluated by flow cytometry method. The results illustrate mean ± SEM. **; P<0.01 compared to the control group.

Article Snippet: Flow cytometry analysis was performed with a FACSCalibur (BD Biosciences) using directly labeled monoclonal Abs (mAbs), PerCP mouse anti-human CD4, FITC mouse anti-human CD25, APC mouse anti-human CD45RA, PerCP-CYTM5.5 mouse anti-human CD73, APC mouse anti-human CD39, PE mouse anti-human FOXP3 (BD Biosciences), APC antimouse/ human Helios (Biolegend, Germany) and isotype control antibodies, all according to the manufacturers’ protocol.

Techniques: Derivative Assay, Flow Cytometry

Journal: eLife

Article Title: Role of distinct fibroblast lineages and immune cells in dermal repair following UV radiation-induced tissue damage

doi: 10.7554/eLife.71052

Figure Lengend Snippet: ( A ) Immunostaining of human skin for CD39 (green) and vimentin (VM) (red) and quantification of double-positive cells per field of view relative to control skin at indicated time points after acute ultraviolet radiation (acUVR) exposure (n = 6 biological replicates). ( B ) Experimental design of mouse acUVR model (top panel), representative images of skin erythema (middle panel), and H&E skin section (bottom panel), showing epidermal hyperplasia and increased dermal cell density 1 day after acUVR. ( C, D ) Representative PDGFRαH2BEGFP sections (green) stained for yH2AX ( C ), and cCasp3 ( D ) (red) of control and treated skin and quantification of double-positive cells at indicated time points post-acUVR. Note that the epidermis and upper dermis show pronounced DNA damage (yH2AX+) with clusters of apoptotic cells (cCasp3+) 24 hr post-acUVB. ( E ) Immunostaining of PDGFRαH2BEGFP back skin (green) for all lymphocytes (CD45; red) and quantification of the CD45 mean fluorescence intensity at indicated time points post-UVR. ( F, G ) Quantification of dermal fibroblast density (PDGFRαH2BEGFP+) ( F ) and total dermal density (DAPI+) ( G ) 24 hr after acUVB in the upper and lower dermis. ( H ) Representative PDGFRαH2BEGFP sections (green) stained for Ki67 (red) of control and treated skin and quantification of double-positive cells at indicated time points post-acUVR. Note that the epidermis and upper dermis show increased proliferation 4 days after acUVB.Nuclei labelled with DAPI and dashed white line delineates upper and lower dermis. Scale bars, 50 μm. Data are mean ± SD. *p<0.05, **p<0.01, ***p<0.001. Source data of shown quantifications are summarised in . Figure 1—source data 1. Source data of quantifications represented as graphs in .

Article Snippet: Antibody , Anti-human CD39 (mouse monoclonal) , eBioscience , Clone eBioA1 (A1) , IF (1:200).

Techniques: Immunostaining, Control, Staining, Fluorescence

Journal: eLife

Article Title: Role of distinct fibroblast lineages and immune cells in dermal repair following UV radiation-induced tissue damage

doi: 10.7554/eLife.71052

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-human CD39 (mouse monoclonal) , eBioscience , Clone eBioA1 (A1) , IF (1:200).

Techniques: Recombinant, Staining, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Software, Imaging

Journal: Cancer Cell

Article Title: Determinants of anti-PD-1 response and resistance in clear cell renal cell carcinoma

doi: 10.1016/j.ccell.2021.10.001

Figure Lengend Snippet:

Article Snippet: Mouse anti-human monoclonal anti-CD39 [TU66; BV711] , BD Biosciences , Cat# 563680; RRID AB_2738369.

Techniques: Staining, Sequencing, Next-Generation Sequencing, Derivative Assay, Methylation, Software